AGAR DIFFUSION PROCEDURES FOR THE ASSAY OF LECITHINASE FROM CLOSTRIDIUM PERFRINGENS
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چکیده
منابع مشابه
Agar diffusion procedures for the assay of lecithinase from Clostridium perfringens.
Lecithinase can be determined by the measurement of the opalescence developing in serum (Nagler, 1939; Seifert, 1939), the precipitation of lecithovitellin (van Heyningen, 1941a; Weed et al., 1942; Theriault, 1945), the release of hemoglobin from erythrocytes (van Heyningen, 1941b; Burrows, 1951), the determination of the amount of acid soluble phosphorus released from lecithin (MacFarlane and ...
متن کاملLocalization of lecithinase activity in Clostridium perfringens.
The lecithinase of Clostridium perfringens is an exoenzyme (3). However, lecithinase activity was demonstrated in cytoplasmic extracts obtained from mechanically disrupted vegetative cells of type A C. perfringens (4). This report deals with the intracellular and extracellular lecithinase activities of vegetative cells and spores of type A C. perfringens. Strain BP6K (1), which produces conside...
متن کاملLecithin-agar assay for lecithinase antibodies in serum.
A technique for assay of lecithinase antibodies in serum was developed in this laboratory by using a lecithin-agar plate diffusion procedure based on a combination of described plate assays. Egg yolk lipoprotein composed primarily of lecithin was used as a substrate for reaction with free or non-neutralized lecithinase C after incubation of known amounts of lecithinase C with various dilutions ...
متن کاملLecithinase activity of mutant and wild strains of Clostridium perfringens.
In a preliminary survey of 30 strains of type A Clostridium perfringens freshly isolated from human feces, soil, and foods, a wide variation in their lecithinase activities was observed (J. A. Schulze, M.S. Thesis, Univ. of Montana, Missoula, Montana, 1968). Other investigators also have noted such variations (1, 2, 5). Variation in lecithinase production by strains of C. perfringens may be the...
متن کاملSerology of the soluble antigens of Clostridium perfringens types A-F by agar-gel diffusion.
Ellner, Paul D. (University of Vermont, Burlington) and Carolyn D. Bohan. Serology of the soluble antigens of Clostridium perfringens types A-F by agar-gel diffusion. J. Bacteriol. 83:284-296. 1962.-A serological study by agar-gel diffusion of the soluble antigens of 39 strains of the Clostridium perfringens group has shown them to be extremely heterogeneous. Strain variation occurred within th...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1959
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.77.4.375-382.1959